Association of Parental Support with Reduced Stereotypy in Children with Autism Spectrum Disorder: A Cross-Sectional Study.

Background and Objectives: To analyze the influence of parental presence and use of risperidone on social interaction and apathy among patients with Autism Spectrum Disorder (ASD). Materials and Methods: Cross-sectional study in a reference center for patients with ASD in a city in northeastern Brazil. The research was carried out using a sociodemographic questionnaire, the Dimensional Apathy Scale, and the Social Communication Questionnaire (SCQ) with the domains of social interaction, language, stereotypy, and communication. The referred questionnaire was answered by the parents or guardians of the children with ASD according to the DSM V criteria. Data were analyzed via independent t-test using the SPSS software version 20. Results: Interviews were conducted with 51 parents/guardians of autistic children with a mean age of 8.8 years (±2.95) and a predominance of males, 34 (66.7%). Of this total, 49 (96.1%) of the children attended school; 40 (78.4%) children were on medication, of which 38 (74.5%) were on risperidone. Those children on risperidone had a higher score on the SCQ scale (p = 0.049) and on the domain of stereotyped behaviors (p = 0.033), which indicated greater impairment. Another statistically relevant variable was the presence of married parents, whereby children who did not have the presence of married parents had a higher average of stereotyped behaviors compared to those who had married parents. Conclusions: The results showed differences in the means of social interactions for children on risperidone, especially regarding stereotyped behaviors. However, it is not possible to state whether this difference was due to the use of risperidone or whether they used risperidone precisely because of these behaviors. Also important was that children who had the presence of married parents showed fewer stereotyped behaviors. There was no difference in apathetic behavior between children.

Chitosan Coating Functionalized with Flaxseed Oil and Green Tea Extract as a Bio-Based Solution for Beef Preservation.

Ecological and safe packaging solutions arise as pivotal points in the development of an integrated system for sustainable meat production. The aim of this study was to assess the effect of a combined chitosan (Ch) + green tea extract (GTE) + essential oil (thyme oil, TO; flaxseed oil, FO; or oregano oil, OO) coating on the safety and quality of vacuum-packaged beef during storage at 4 °C. An optimized bio-based coating formulation was selected (2% Ch + 2% GTE + 0.1% FO) to be applied to three fresh beef cuts (shoulder, Sh; knuckle, Kn; Striploin, St) based on its pH (5.8 ± 0.1), contact angle (22.3 ± 0.4°) and rheological parameters (viscosity = 0.05 Pa.s at shear rate > 20 s-1). Shelf-life analysis showed that the Ch-GTE-FO coating delayed lipid oxidation and reduced total viable counts (TVC) and Enterobacteriaceae growth compared with uncoated beef samples over five days. In addition, Ch-GTE-FO coating decreased total color changes of beef samples (e.g., ∆E* = 9.84 and 3.94, for non-coated and coated Kn samples, respectively) for up to five days. The original textural parameters (hardness, adhesiveness and springiness) of beef cuts were maintained during storage when Ch-GTE-FO coating was applied. Based on the physicochemical and microbial characterization results, the combination of the Ch-GTE-FO coating developed was effective in preserving the quality of fresh beef cuts during refrigerated storage along with vacuum packaging.

Longitudinal behavior of orthodontic extraction spaces: A retrospective dental cast analysis.

INTRODUCTION: This retrospective study aimed to qualitatively and quantitatively evaluate orthodontic extraction space behavior and size at the end of treatment and the short- and long-term stages in Class I, II, and III malocclusions. METHODS: The sample comprised records of 1283 orthodontic patients treated with extractions (cross-sectional group). From these patients, 1- and 5-year posttreatment records of 214 patients were additionally evaluated (longitudinal group). Orthodontic extraction sites of immediate (end of treatment), short-term (1-year posttreatment), and long-term (5-year posttreatment) stage dental casts were measured with a digital caliper. Extraction spaces were classified according to their qualitative behavior in the open, closed, reopened, and recently closed spaces. Space qualitative longitudinal behavior was compared among the malocclusion types with chi-square tests. Kolmogorov-Smirnov normality tests were performed to check the variable's distribution. All variables showed normal distribution, except the malocclusion group ages. Therefore, parametric tests were used for all comparisons between the malocclusion groups, except for age comparisons in each stage, which were compared with the Kruskal-Wallis tests. Thus, space size and its reduction were compared among the 3 stages, in general, and between the maxillary and mandibular arches, with analysis of variance followed by Tukey tests and t tests, respectively. RESULTS: Classes I, II, and III malocclusion patients presented similar ages at each stage. At the end of treatment, 71.4% and 65.1% of the quadrants were closed in the cross-sectional and longitudinal groups, respectively, and 43.6% and 38.2% of patients presented all quadrants closed at this same stage in both groups. The reopening and late closure tendencies were 2.9 and 2.3 times greater in the short term than in the long term. Reopening tendency in the short-term was greater in Class II than in Class I malocclusion. Of the quadrants closed at the end of treatment, 87% were stable in the long term. From the quadrants with residual spaces at the end of treatment, 28.4% persisted openly in the long term. Residual space size reduced significantly in the short and long term, primarily in the mandibular arch. CONCLUSIONS: The percentage of patients with residual spaces at the end of treatment was 56.4%. Space reopening and late closure occur mainly in the first year after treatment. There was long-term stability in 87% of the quadrants closed at the end of treatment. There was a significant reduction in orthodontic extraction space size in the short and long term, especially in the mandibular arch.

Avaliação da terceira etapa do método canguru na atenção primária a saúde / Evaluation of the third stage of the kangaroo method in primary health care / Evaluación de la tercera etapa del método canguro en atención primaria de salud

Objetivo: avaliar a assistência prestada às crianças pela Atenção Primária à Saúde na terceira etapa do método canguru. Métodos: estudo transversal com 156 crianças pré-termo no período de janeiro a junho de 2020. Utilizou-se questionário contendo variáveis sócio-demográficas, clínicas e relacionadas à assistência prestada. Resultados: Dentre as crianças: 54,5% tinham idade acima de seis meses; 51,9% eram do interior ou de outras localidades; 94,9% eram de baixa renda; 39,1% tiveram peso menor que 1500g; 62,2% e 12,2% nasceram com idade gestacional entre 28 a 33 semanas e inferior a 28 semanas, respectivamente. Quanto à assistência prestada: 74,4% das crianças não realizaram consultas na atenção primária e dentre as atendidas, 78,2% classificaram como ruim/regular; 59,6% não receberam visita domiciliar. Conclusão: a avaliação da terceira etapa do método canguru na atenção primária, sob o olhar de mães, apontou fragilidades na qualificação, integralidade da assistência, redesenho/discussão da rede, na referência e contra-referência.

Objective: to evaluate the assistance provided to children by Primary Health Care in the third stage of the kangaroo method. Methods: cross-sectional study with 156 preterm children from January to June 2020. A questionnaire was used containing socio-demographic, clinical and care-related variables. Results: Among the children: 54.5% were over six months old; 51.9% were from the countryside or other locations; 94.9% were low-income; 39.1% weighed less than 1500g; 62.2% and 12.2% were born with gestational age between 28 and 33 weeks and less than 28 weeks, respectively. As for the assistance provided: 74.4% of the children did not undergo consultations in primary care and among those attended, 78.2% classified it as poor/fair; 59.6% did not receive a home visit. Conclusion: the evaluation of the third stage of the kangaroo method in primary care, from the perspective of mothers, pointed out weaknesses in qualification, comprehensiveness of care, network redesign/discussion, in reference and counter-reference.

Objetivo: evaluar la asistencia brindada a los niños por la Atención Primaria de Salud en la tercera etapa del método canguro. Métodos: estudio transversal con 156 niños prematuros de enero a junio de 2020. Se utilizó un cuestionario con variables sociodemográficas, clínicas y asistenciales. Resultados: Entre los niños: 54,5% tenían más de seis meses; El 51,9% eran del campo u otras localidades; 94,9% eran de bajos ingresos; El 39,1% pesaba menos de 1500 g; El 62,2% y el 12,2% nacieron con edades gestacionales entre 28 y 33 semanas y menos de 28 semanas, respectivamente. En cuanto a la asistencia brindada: el 74,4% de los niños no acudió a consultas en atención primaria y entre los atendidos, el 78,2% la clasificó como mala / regular; El 59,6% no recibió visita domiciliaria. Conclusión: la evaluación de la tercera etapa del método canguro en atención primaria, desde la perspectiva de las madres, señaló debilidades en la calificación, integralidad de la atención, rediseño / discusión de la red, en referencia y contrarreferencia.

Ressignificação da cultura no contexto da Saúde da Mulher: como a Antropologia subsidia as ciências médicas? / Reframing culture in the context of women's healthcare: how does Anthropology support medical science?

A Antropologia moderna entende a cultura como a construção de uma série de elementos simbólicos ao longo da vida pelos indivíduos (conceito importante para a superação de determinismos vigentes anteriormente). Atualmente, as ciências médicas são entendidas também como parte das dinâmicas sociais. Desta forma, grupos historicamente minoritários por sua marginalização social, como as mulheres, encontram uma lógica muito semelhante no campo da saúde. A Saúde da Mulher pouco humanizada e não centrada na paciente é um problema ainda não superado no campo das ciências médicas, sendo uma batalha sobretudo de cunho social. É importante que as mulheres se apropriem da linguagem técnico-científica que rege o mundo contemporâneo e sejam também desmanteladoras de armadilhas sociais que estão constantemente agindo no subjugo de minorias sociais. A Antropologia moderna, como instrumento de ressignificação da cultura e de luta pela superação de problemas sociais, é um importante subsídio para as ciências médicas.

Modern Anthropology understands that culture is the construction of a series of symbolic elements throughout the lifespan of an individual (important concept to overcome previously existing determinisms). Medical science is also currently understood as being a part of social dynamics. Therefore, groups that are historically considered minority due to social marginalization, including women, encounter a very similar logic in the field of healthcare. Failure to be humanized and patient-centered in women's healthcare is a problem that remains to be overcome in the field of medical science. Furthermore, it is a battle of a social nature, in particular. The appropriation of technical-scientific language governing the contemporary world by women is also important to dismantle the social traps which are constantly acting to subdue social minorities. Modern Anthropology plays an important role in medical science, as a tool for reframing culture and struggling to overcome social problems

Efficacy and adverse event profile of cannabidiol and medicinal cannabis for treatment-resistant epilepsy: Systematic review and meta-analysis.

This paper aimed to systematically examine the efficacy and adverse event (AE) profile of cannabidiol and medicinal cannabis by analyzing qualitative and meta-analytic data. We used the terms ("Cannabidiol" OR "Cannabis") AND "Epilepsy" AND ("Treatment" OR "Therapeutics") as keywords to retrieve studies indexed on PubMed, ScienceDirect, and CENTRAL databases. The inclusion criteria were as follows: clinical studies with a longitudinal observational design and intervention using cannabinoid derivatives, especially cannabidiol and medicinal cannabis, whereby some results involved the frequency of epileptic seizures. We used Cochrane Collaboration's Review Manager software (RevMan 5.1.6) for the meta-analysis and dichotomized the articles to a confidence interval of 95%. From 236 articles, we selected 16 for descriptive analysis; we selected only 4 for the meta-analysis. According to the results, a statistically meaningful effect of cannabidiol compared with placebo was observed (p < 0.00001). When comparing treatment with cannabidiol or medicinal cannabis, significance was not found for the AE profile (p = 0.74). As AEs for cannabidiol were more common under short-term than under long-term treatment (p < 0.00001), this approach was favorable in the long term. Furthermore, cannabidiol is more effective than placebo, regardless of the etiology of epileptic syndromes and dosage. Overall, the AE profile did not differ across treatments with cannabidiol or medicinal cannabis, though it did differ favorably for long-term than for short-term treatment.

Long-term profile attractiveness in Class II Division 1 malocclusion patients treated with and without extractions.

INTRODUCTION: This study evaluated the influence of Class II treatment protocols in profile attractiveness. METHODS: Sixty-eight patients with initial full Class II Division 1 malocclusion, orthodontically treated an average of 15 years before, and matched by sex, age, time after treatment, orthodontic outcomes, and overjet, were compared. Three groups were formed, according to the treatment protocol: NE, nonextraction (n = 20; mean age 29.94 years, 15.62 years after treatment); 2E, 2-premolar extractions (n = 27; mean age 30.56 years, 15.09 years after treatment); and 4E, 4-premolar extractions (n = 21; mean age 32.29 years, 17.20 years after treatment). Cephalometric measurements and profile silhouettes were obtained from posttreatment and long-term posttreatment lateral cephalograms. With a 10-point numeric scale, 77 orthodontists and 77 laypeople rated profile attractiveness of each silhouette on a website. RESULTS: The raters' posttreatment and long-term posttreatment scores, respectively, were: NE 4.76 and 4.32; 2E 5.35 and 5.08; and 4E 4.53 and 4.33. CONCLUSIONS: The posttreatment profile attractiveness was significantly higher in the 2E than in the 4E group, and in the NE group it was similar to the others. The long-term profile attractiveness in the 2E group was significantly greater compared with the NE and 4E groups.

Cashew Nut Allergy: Clinical Relevance and Allergen Characterisation.

Cashew plant (Anacardium occidentale L.) is the most relevant species of the Anacardium genus. It presents high economic value since it is widely used in human nutrition and in several industrial applications. Cashew nut is a well-appreciated food (belongs to the tree nut group), being widely consumed as snacks and in processed foods by the majority of world's population. However, cashew nut is also classified as a potent allergenic food known to be responsible for triggering severe and systemic immune reactions (e.g. anaphylaxis) in sensitised/allergic individuals that often demand epinephrine treatment and hospitalisation. So far, three groups of allergenic proteins have been identified and characterised in cashew nut: Ana o 1 and Ana o 2 (cupin superfamily) and Ana o 3 (prolamin superfamily), which are all classified as major allergens. The prevalence of cashew nut allergy seems to be rising in industrialised countries with the increasing consumption of this nut. There is still no cure for cashew nut allergy, as well as for other food allergies; thus, the allergic patients are advised to eliminate it from their diets. Accordingly, when carefully choosing processed foods that are commercially available, the allergic consumers have to rely on proper food labelling. In this sense, the control of labelling compliance is much needed, which has prompted the development of proficient analytical methods for allergen analysis. In the recent years, significant research advances in cashew nut allergy have been accomplished, which are highlighted and discussed in this review.

Avaliação do conhecimento de médicos intensivistas de teresina sobre morte encefálica / Evaluation of the knowledge of intensive care doctors in teresina concerning brain death / Evaluación del conocimiento de los médicos intensivistas de teresina respecto a la muerte cerebral

Trata-se de estudo transversal e descritivo, realizado a partir de questionário respondido por 90 médicos atuantes em unidades de terapia intensiva de Teresina, Piauí, Brasil. Objetivou avaliar o conhecimento dos médicos intensivistas sobre morte encefálica e correlacionar esse dado com tempo de exercício da profissão, tempo de atuação em UTI, tipo de unidade em que o profissional trabalha, curso de residência médica e posse de título de especialista em terapia intensiva. Os participantes demonstraram, em sua maioria, conhecer a definição de morte encefálica, com melhores resultados entre aqueles com menor tempo de exercício da profissão médica. Demonstraram saber da obrigatoriedade de exames complementares para o diagnóstico de morte encefálica e descreveram-se como seguros ou muito seguros para explicar morte encefálica a familiares de pacientes. Os médicos, de modo geral, demonstraram dificuldades em determinar o horário legal do óbito por morte encefálica de paciente considerado doador de órgãos.

A descriptive study was carried out based on a questionnaire answered by 90 doctors working in the intensive care units of Teresina, Piaui, Brazil. The aim of the study was to evaluate intensive care knowledge on brain death and correlate it with time spent working in the medical profession, time spent working in intensive care units, type of unit in which the medical professional spent their Medical Residency course and whether the medical professional had the title of specialist in Intensive Care. The majority of participants demonstrated knowledge of the definition of brain death, and awareness was greater among those who had spent less time working in the medical profession. They demonstrated knowledge of the requirement for additional tests to diagnose brain death and described themselves as confident or very confident when explaining brain death to the relatives of patients. The doctors, in general, had difficulties in determining the legal time of death of patients with brain death who were classed as organ donors.

Este es un estudio transversal y descriptivo, realizado a través de un cuestionario del que participaron 90 médicos que actúan en las unidades de terapia intensiva de Teresina, Piauí, Brasil. Se realizó con el objetivo de evaluar el conocimiento de los médicos intensivistas sobre la muerte cerebral y correlacionarlo con el tiempo de ejercicio de la profesión médica, tiempo de actuación en unidades de terapia intensiva, tipo de Unidad en la cual el profesional trabaja, carrera de Residencia Médica y posesión del título de especialidad en Terapia Intensiva. En general, los participantes demostraron conocer la definición de muerte cerebral, siendo este conocimiento mayor entre aquellos con menor tiempo de ejercicio de la profesión médica. Demostraron conocer la obligatoriedad de los exámenes complementarios para diagnosticar la muerte cerebral y se describieron a sí mismos como seguros o muy seguros al momento de explicar la muerte cerebral a los familiares de los pacientes. De modo general, estos médicos presentaron dificultades para determinar el horario legal del óbito en pacientes con muerte cerebral cuando se trata de donantes de órganos.

Antibacterial activity tannin-rich fraction from leaves of Anacardium humile / Atividade antibacteriana de frações tânicas de folhas de Anacardium humile

In vitro tests conducted with extracts rich in tannins have identified several biological activities of this class of substance. Thus, this paper intends to evaluate the antibacterial activity of tannin-rich fraction obtained from leaf extracts of Anacardium humile A.St.-Hil. Extracts of A. humile leaves in 70% acetone were semi-purified with ethyl acetate and butanol. We quantified the total tannins of the semi-purified fractions, of the crude extract and of aqueous residues and then performed tests of the antibacterial activity of the tannins against Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 27853 and Enterococcus faecalis ATCC 29212. All positive extracts underwent tannin isolation using a Sephadex LH-20 column. The tannins isolated from the samples were quantified and tested for the minimal inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). The tannins from crude extracts, semi-purifications and residues showed inhibition of S. aureus growth with MIC=500 g mL-1. All tannin fractions showed MIC against all strains and MBC, except against E. faecalis. The tannin fractions from Anacardium humile have antibacterial activity against Staphylococcus aureus, Pseudomononas aeruginosa and Enterococcus faecalis and, therefore, may be promising for future synthesis of new antibacterial agents.

Testes in vitro realizados com extratos ricos em taninos têm identificado muitas atividades biológicas dessa classe de substâncias. Nesse contexto, esse artigo propõe a avaliação da atividade antibacteriana de frações tânicas, obtidas de extratos de folhas de Anacardium humile A.St.-Hil. Extratos de A. humile em acetona 70% foram particionados com acetato de etila e butanol. Foram quantificados taninos totais das frações particionadas, do extrato bruto e dos resíduos aquosos, e foi realizado teste de atividade antibacteriana dos taninos contra Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 27853 e Enterococcus faecalis ATCC 29212. Todos os extratos positivos foram submetidos ao isolamento de taninos, utilizando-se coluna de Sephadex LH-20. Os taninos isolados das amostras foram quantificados e testados sua concentração inibitória mínima (CIM) e concentração bactericida mínima (CBM). Os taninos do extrato bruto, partições e resíduos apresentaram inibição do crescimento de S.aureus com CIM =500 g mL-1. As frações tânicas apresentaram CIM contra todas as cepas e CBM, exceto contra E. faecalis. As frações tânicas de Anacardium humile possuem atividade antibacteriana contra Staphylococcus aureus, Pseudomononas aeruginosa e Enterococcus faecalis e, portanto, podem ser promissoras em sínteses futuras de novos agentes antimicrobianos.